It is a red stain that is widely used as a counterstain in Gram staining and endospore staining methods.Haematoxylin and eosin (H&E) is often used together for screening cell structure and shape in a tissue sample, helping in diagnosis of diseases like cancer.It is used as a counterstain to Haematoxylin.It is used to stain cytoplasm, RBCs, collagen and muscle fibres.
It is a class of pinkish-red fluorescent dye.Staining Mechanism - It produces a blue dye called Hematin by oxidation reaction with nuclear histone proteins, causing the nucleus to appear purple-blue.Origin - Heartwood of Haematoxylum campechianum.Haematoxylin is a commonly used nuclear stain.The bulk content of the nucleus is nucleic acid and so, nucleic acid stains are used as nuclear stain.Nuclear stains are used to stain the cell nucleus specifically.Different types of stains are used for staining different cells and tissues of an organism.
Thus staining them gives better visibility under microscopes. Cells and cellular structures are generally colourless and transparent. Auxin is used in tissue culture to regulate rooting and shooting of plants. It is a plant hormone that regulates apical dominance, cell division, elongation and differentiation. It is used for encapsulation of somatic plant embryos that are used as seeds. It is used in the formation of synthetic seeds. It is also used to induce osmotic stress in tissue culture-raised plants. It is used in plant tissue culture for fusion of protoplast of two different cells. Allopolyploidy - increase in chromosomes number, from two different species, e.g., AABBĪdditional Information PEG (polyethylene glycol). Autopolyploidy - repetition of the same set of chromosomes, e.g., BBB. Cell having three or more sets of chromosomes. Poly means 'many' and ploidy means 'haploid set of chromosomes'. More than the normal two sets of chromosomes. Raphano brassica is sterile which after colchicine application becomes fertile with 4n = 36. Raphanus sativus (2n = 18) × Brassica oleracea (2n = 18) = Raphano brassica (2n = 18). Results in doubling of chromosomes number in progeny. So, the sister chromatids do not segregate during anaphase. It suppresses the mitotic spindle fiber formation. It prevents microtubule formation during cell division. It is used to induce polyploidy in plants. Colchicine is an alkaloid from the seeds of Colchicum autumnale.
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